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TLR genes of buffalo, goat, mithun and yak would be amplified and sequenced to determine how these genes have evolved among different animal species.
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The basal expression levels of TLR mRNAs of three distinct breeds of buffaloes and goats would be assessed using real time PCR to determine whether these species / breeds are susceptible / resistant to pathogens by virtue of their reduced or enhanced levels of TLR mRNAs
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If enhanced levels of TLR mRNAs are found in some breeds, then the reason for such enhancement would be assessed by screening for any favourable mutations (SNPs) in their coding genes or promoter regions.
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The ligands for different TLRs and their induced cytokine profiles would be assessed in buffalo and goat immune cells to identify suitable ligands for therapeutic interventions
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The role of TLR genes in conferring species-specific disease resistance would be further confirmed using disease resistant models wherein buffalo and goats are either susceptible or resistant to bacterial, viral and parasitic diseases.
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